8/9/2023 0 Comments Seashore paspalum![]() Analyses by PCR confirmed that hpt and bar genes were successfully expressed in the respective transformed seashore paspalum materials. Through screening multiple resistant calli and resistant regenerated seedlings, we obtained regenerated transgenic plants expressing each vector. L -1 acetosyringone and 0.01% Silwet L-77, followed by vacuum treatment for 10 mins, then soaking for 30 mins and co-culturing for 2 days.The optimal transformation conditions were as follows: ultrasonic treatment of the explant for 20 min with bacterial solution (concentration OD 600=0.6) containing 100 μmol Calli subcultured for 24 weeks were selected for Agrobacterium-mediated genetic transformation. The optimal concentration of glufosinate for screening was 1.2 mg The optimal concentration of hygromycin for screening was 120 mg The results showed that the regeneration rate of callus decreased significantly after subculture for 36 weeks, and the highest regeneration rate was 67.9% after subculture for 48 weeks. After optimizing the genetic transformation conditions, we compared the transformation efficiency of hpt and bar genes as selection markers in Agrobacterium-mediated transformation of seashore paspalum. To establish an efficient and stable genetic transformation system for this plant, we used its mature seeds as explants, and determined the optimal selection pressures of hygromycin and glufosinate during callus subculture and the regeneration stage. Seashore paspalum is a warm-season turfgrass with strong salt tolerance, and it has a wide range of potential applications.
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8/9/2023 0 Comments Mysimbl not working![]() ![]() To install mySIMBL, download the application from the project’s GitHub page. By itself, it doesn’t change any functionality, but it provides the tool that allows you to install plugins. Installing mySIMBL on macOSīy itself, mySIMBL is only a framework for managing plugins. Fundamentally, you can imagine SIMBL as a package manager for application plug-ins. ![]() It can inject changes into other applications, allowing you to change how applications look and run. Today, SIMBL is a platform for modifying how programs run on your Mac. Originally, SIMBL was used to modify Apple’s Safari browser (once known as PithHelmet), which didn’t have an extension infrastructure until 2010. It allows developers to make modifications to programs written in Cocoa without access to the source code. Simple Bundle Loader and Smart Input Manager Bundle Loader) is the basis for mySIMBL. If these projects become successful, this will provide people who desire the early Mac OS X experience modern systems that will maintain that experience.SIMBL (a.k.a. Both projects are based on a FreeBSD foundation, but the major difference between the projects is airyxOS is a much more ambitious attempt to reimplement macOS's infrastructure (even going as far as to aim for supporting "trivial" Cocoa applications), while helloSystem has different (Qt) underpinnings, with an emphasis on replicating the Mac OS X look-and-feel and promoting adherence to the Macintosh Human Interface Guidelines. I am keeping an eye on two very interesting projects that attempt to replicate the spirit of early Mac OS X: helloSystem ( ) and airyxOS ( ). I miss macOS, but I enjoy the openness of PCs, and I enjoy the flexibility of Windows and FreeBSD. Due to my disappointment with Apple's direction (especially since roughly 2016), I opted not to upgrade my aging 2013 MacBook Air and 2013 Mac Pro with new Macs, instead switching to a Microsoft Surface Pro (running Windows 10) and a custom Ryzen 3900X build (which runs both Windows 10 and FreeBSD). The user interface also gradually started adopting more iOS influences, which I think take away from the desktop experience. Then came the Tim Cook era, and with it came the gradual locking down of the Mac, both in terms of hardware (for example, the soldering of formerly upgradable components such as RAM and storage) and software (for example, notarization). It felt much more pleasant than Windows of the era (though I admit I liked Windows 7), and the desktop environments for Linux and the BSDs simply didn't compare. It was a nice marriage of NeXT technology and an updated version of the venerable Macintosh user interface. In my opinion Mac OS X peaked at Snow Leopard in fact, I'd be comfortable using Snow Leopard (or even Tiger) as my daily driver today if it supported current hardware and if there were a modern web browser for it. It also provided me a Unix shell whenever I needed it. It had a well-designed user interface, and most applications conformed to the Apple Human Interface Guidelines. Mac OS X in the 2000s to me was heads-and-shoulders better than the competition. I've remained a Mac OS X user from Tiger all the way to Mojave. I switched from a Windows XP/FreeBSD dual boot configuration to Mac OS X Tiger back in 2006 when I bought my first modern Mac, a Core Duo MacBook. My feelings about Mac OS X are similar to the author's. 8/9/2023 0 Comments Utopia series 1 buyThe Utopia season 2 finale was a fantastic set-up for the future, but by absolutely no means did Utopia's ending provide resolution. A brand new villain emerged, a redemption arc was teased for an old adversary, and the main cast were last seen being captured by the bad guys. Not only was the overarching plot of Utopia left on a knife edge, but the fates of the main cast remained in limbo. Utopia fans were understandably frustrated by this decision. Dennis Kelly has since speculated that releasing in summer didn't help Utopia's chances, while its director suggested that the London-heavy setting might've played a part in deterring a wider audience. Indeed, despite rave reviews and a loyal, passionate following, Utopia season 2's viewership dipped consistently below the 1 million mark, with the winding story, uncomfortable themes and unique style perhaps not suited to retaining casual viewers. In late 2014, the broadcaster wrote that ending Utopia was " a necessary part of being able to commission new drama." In other words, Utopia didn't attract large enough viewing figures to warrant its place on Channel 4's 2015 schedule. Unfortunately, fans eagerly anticipating Utopia season 3 were left bitterly disappointed when Channel 4 announced that Utopia had not been renewed for another run. Related: Rainn Wilson Roles: Where You Recognize The Office Star ![]() A second series aired in 2014 to further critical acclaim, and ended on an almighty cliffhanger. Utopia was praised for its intelligent thriller-style plot and stunning cinematic visuals, but it was also searingly brutal and unflinchingly violent, earning a few complaints from disgruntled viewers and concerned members of society. ![]() Created by Dennis Kelly, Utopia aired in 2013 with a premise virtually identical to the remake. There's considerable buzz and a big marketing push behind Amazon's Utopia remake, but the situation was quite different for the original UK series that inspired it. ![]() The collection grew over the years with Bison in 1963, forming the first privately owned Bison in the state of Tennessee. This was a pivotal moment in the young mans life and he knew he wanted to see animals from around the world saved and protected right here on the family farm. They collected pheasants, peafowl and various types of deer. Claude H Conley I instilled a strong interest in his son, Claude M Conley, at a very young age to love and appreciate Hillcrest, the Conley Family and all of its animals. The farm has produced numerous types of crops and has always stayed entrepreneurial in the products that were developed and sold on the farm. The Tennessee Safari Park is located on a century farm owned by the Conley Family since 1858, called Hillcrest. We aim to gain long term sustainability for our world's rare and endangered flora and fauna in their natural environment. The Tennessee Safari Park's mission is to create and provide educational activities and environments for the community and abroad to bring appreciation and conservational awareness for all living things. Our large gift shop offers a wide variety of souvenirs, gifts, drinks, and snacks. ![]() A gem mining sluice is open seasonally for kids of all ages to enjoy. ![]() This exhibit is seasonal due to the temperature requirements of the animals however, we have alternate viewing options for colder months. Watch these curious and always on the go creatures enjoying their exhibit as they travel to and fro in an open air tunnel that goes right over guests’ heads! Immerse yourself in our newest exhibit, "the sloth barn," which brings you a spectacular panoramic view of amazing animals from South America! We have a large group of Linnaeus's two-toed sloths and the equally odd and adorable prehensile-tailed porcupines. You can also hand feed the parakeets and some may even land on your hand, arm, shoulder, or even your head! Our squirrel monkey exhibit is another guest favorite. Some daring guests even hold the carrot in their mouth to feed the giraffe which results in an amazing experience, photo, and usually a slobbery kiss from an 18inch long tongue! Parakeet landing is where you can see free flight parakeets, Australian crested doves, and demoiselle cranes. Our giraffe feeding station allows you to hand feed a giraffe and be sure to take a selfie to capture the moment. If feeding a giraffe is on your bucket list you're in the right place. We offer a petting zoo of adorable and friendly pygmy goats for more hands on fun to enjoy. Our walk through zoo allows you to get out and stretch your legs along 20 acres of additional animal exhibits and encounters. It enriches their daily lives as well as ours. These animals enjoy free range freedom but can usually be seen choosing to visit with our guests. ![]() Some will even block your path to make sure you stop and say “hi”. Why be on the outside looking in like a typical zoo when you can drive right in and join them? As you drive along and explore our drive through safari, animals from every continent but Antartica will come right up to your vehicle windows. Our drive through safari offers the unique experience of close up interactions with exotic animals as well as actually feeding them yourself. The Tennessee Safari Park boasts a 7.5 mile drive through safari and a 20 acre walk through zoo. The manicotti was quite a delight and the Italian salad was great too. Restaurants near Westport Inn, Joplin on Tripadvisor: Find traveller reviews and candid photos of dining near Westport Inn in Joplin, Missouri. CLUB 1201 - 41 Photos & 33 Reviews - 1201 E 32nd St, Joplin, MO - Menu - Yelp Club 1201 34 reviews Claimed $$ Caterers, Breakfast & Brunch, Sandwiches Open 9:00 AM - 2:00 PM See hours Finn’s Sami’s Family. Craigslist Tulsa Atvs For Sale By OwnerCraigslist - ATVs for Sale in Little Rock, AR: John Deere 2007 Gator HPX, Kawasaki 2008 Mule 4x4, Kubota 20 4x4, Yamaha RIDE WITHOUT COMPROMISE Push the limits with a CFMOTO ATV. Fast Food Restaurant No GF Menu GF menu options include: Burgers, Fries 100% of 7 votes say it's celiac friendly 3.1749 S Campbell Ave, Springfield, MO 65807 Closed1801 S Range Line Rd, Joplin, MO 64804 $ Specialties: Your Hugoton, KS Casey's at 1034 S Main St has the best pizza featuring made-from-scratch dough, real mozzarella cheese, and only the freshest pizza toppings. With exceptional, outstanding service and succulent burgers, The Eagle Drive-In is a diner that specializes in New American and traditional fare, soul food, big portions and big flavors. IN BUSINESS (417) 659-8686 2825 E 32nd St Joplin, MO 64804 CLOSED NOW good food-good prices. ![]() If you're looking for a premium all wood storage shed kit or Amish shed, All Pre-Cut Best Barns sheds, EZ-Fit sheds, Handy Home Products sheds and Palmako sheds are excellent. If you're ready to increase customer retention and grow your bottom line, take a look at what Total Touch® POS can do for you. M&M Bistro is a Mediterranean cuisine in heart of Downtown Joplin with variety of food from Greece, Spain. Featuring a casual yey cozy atmosphere, Broadway Burgers & More took away 4. Logan's Roadhouse Restaurants American Restaurants Barbecue Restaurants (3) 6. Red Onion Cafe 659 reviews Open Now American, SHARE Carmine's Pizza NEW YORK STYLE PIZZA See profile WHERE ARE WE EATING. Women's Apparel-Retail: Fashion Bug, 2405 W 7th St.This is our guide to the best places to eat in Joplin, Missouri. Video Tapes & Discs-Renting & Leasing: Crown Video, 1721 W 7th St. Variety Stores: Dollar General, 807 W 7th St. Trucks-Industrial (Wholesale): United Machinery & Supply Co, 2002 W 7th St. Tax Return Preparation & Filing: H & R Block Tax Svc, 1651 W 7th St # 4 Services Nec: Systems & Svc Technologies Inc, 1717 W 7th St. Schools: West Central Elementary School, 1001 W 7th St. Subway Sandwiches & Salads, 2623 W 7th St.Granny Shaffer's Family Rstrnt, 2207 W 7th St # 4.Crabby's Seafood Bar & Grill, 815 W 7th St.Recreation Centers: Pappy's Arcade, 1800 W 7th St. Modeling Schools: Kay Beauty Salon, 1201 W 7th St. Metal Fabricators: Rantec Fabrication, 2000 W 7th St. Grocers-Retail: Save-A-Lot, 811 W 7th St. Gift Shops: Amanda's Hallmark Shop, 1651 W 7th St. Garbage Collection: WCA Waste Corp, 3700 W 7th St. Floor Materials: Ridout Interior Design, 2500 W 7th St. Electric Contractors: Crane Electric Heating & Air, 430 W 7th St. Department Stores: Wal-Mart Supercenter, 2623 W 7th St. Convenience Stores: Snak-Atak, 2307 W 7th St. ![]() ![]() Contractors-Equipment & Supls-Renting: Joplin Rental Ctr, 2115 W 7th St.
In the late 1950s, I Love Lucy star and Desilu co-owner Desi Arnaz, husband of Lucille Ball, approached Stack to star as famous 1930s lawman Eliot Ness in The Untouchables. Robert Stack with Bruce Gordon in The Untouchables in 1962. Desi Arnaz asked Robert Stack to take on the role of Eliot Ness. ![]() The film helped kick off the brief 3D movie craze of the 1950s that led to audiences wearing polarized lenses, often for horror films like André De Toth's House of Wax (1953). Though stereoscopic films that created an illusion of depth had been in circulation since the turn of the 20th century, 1952’s Bwana Devil is believed to have been the first feature-length 3D color movie, a feat accomplished by using technology dubbed "Natural Vision." In Bwana Devil, Stack plays Jock Howard, a railroad worker desperate to capture the man-eating lions threatening the construction of a railroad in Africa. Robert Stack was in the very first 3D movie. When he returned to work following the war, Stack settled into his now-familiar screen persona of a strong authority figure. While these early roles-even as a member of the Reich-featured Stack’s boyish demeanor, serving as a gunnery officer and instructor during World War II shaved much of the adolescent charm off his screen presence. Other roles followed, including one as a Nazi in 1940’s The Mortal Storm and 1942’s To Be or Not to Be. Because of Durbin’s fame, the romantic interlude created a lot of publicity for Stack. Stack made his film debut in 1939’s First Love, giving popular teen actress Deanna Durbin her first onscreen kiss. ![]() Robert Stack with Wanda Hendrix in 1951's My Outlaw Brother. World War II changed Robert Stack’s career. “If you kick people around,” Gable told him, “I’m going to kick you.” 3. Later, actor and family friend Clark Gable encouraged Stack to get into acting and to use any power or influence drawn from the profession to help people. While standing off to one side of the stage at a talent show, a talent scout for Universal approached Stack and signed him to a studio contract. At USC, Stack supplemented his sports pursuits with drama classes, giving him his first taste of performing. At 16, Stack was a member of the All-American Skeet Rifle Team, setting two world records and becoming the National Skeet Champion. ![]() He was on the school’s polo team and had also established himself as a national champion in skeet shooting, the sport that involves using firearms to target clay skeets. In high school, and later while attending the University of Southern California, Stack was heavily involved in athletics. Robert Stack was a national skeet shooting champion. English was his third language, one he didn’t learn until he was 7 years old after returning to California when his parents had reconciled. There, he learned both French and Italian as a child. After his parents divorced when he was 3 years old, he remained with his mother and moved with her to Europe so she could study opera. Robert Langford Stack was born in Los Angeles on Januto father James and mother Elizabeth, Stack was a fifth-generation Californian but became a young man of the world early. 8/8/2023 0 Comments Plots matlab vs pythonIn the process of understanding how the code you want to work with works you will get a better understanding how to write good Python code. Reworking the code into idiomatic Python almost invariably ends up with code a fraction the length and considerably faster.Ī good approach, once you get a feel for the basic syntax, would be to look at existing major open-source Python projects you are interested in and contributing some features you want to those. When I look at the code, they tried to shoe-horn MATLAB coding practices into Python. I can't count the number of times I have seen people complaining that Python code is slower and more complicated than the MATLAB code they started with. That is not to say that starting with your existing MATLAB code is necessarily bad, but it isn't going to teach you how to make good use of Python's features. That is almost never the right way to do loops in Python. For example, in MATLAB you pretty much have to loop over indices when looping over arrays, due to the bizarre way MATLAB handles loops. Python can do the same stuff MATLAB can do, but there are often much better ways to do things that aren't possible or feasible in MATLAB. So what do you guys suggest to use compared to those subjects and future projects related to electronics and computer engineering.īe careful with that approach. They didn’t teach us python as I learned python on my own.
![]() ![]() Finally, there are a few different products that are part of this package including Chief Architect Premier, Chief Architect Viewer, Home Designer Professional and Chief Architect Lite.Īt Cad Crowd, we have hundreds of designers prepared to work with you at any time. As we already said, DIY remodelers can use it too. Even educators use this program to help students. On the other hand, interior designers use it for baths, kitchens, and interior design. For instance, architects, builders, and remodelers use it for 3D rendering, framing and CAD, plan & foundations, construction documents and roofs and ceilings. But if you want to feel all the benefits of using Chief Architect, you will need an experienced professional that offers Chief Architect services.ĭifferent people use Chief Architect in different ways. Of course, as we said before, Chief Architect is now one of the favorite home design tools for homeowners, contractors, and builders. Today, designers can use Chief Architect to get the most from modern technology and create designs in a simple and smooth manner in both 3D and 2D rendering environments. According to most experts, this software is one of the most important examples of Building information Modeling (BIM) software solutions because it can be used for residential construction.Ĭhief Architect was introduced to the market back in 1992 when it was used as 3D PC graphics software available to Microsoft Windows users. In addition, it is also used for DIY home projects too. To put it simply, this software is used when someone needs accurate 3D architectural designs. Avoid wasting time on subpar online hiring offers and connect with top-notch Chief Architect freelancers from Cad Crowd right now.Ĭhief Architect Software, or simply Chief Architect, is well-known CAD (computer aided design) software focused on the home building industry. We have designers that are carefully picked, trained, qualified and experienced and we are quite sure that all your Chief Architect needs will be taken care of in a proper way. Our main goal is to link every client to a freelance Chief Architect professional capable of designing the best homes and buildings for their project. Cad Crowd is the one-stop marketplace for all those interested in using top talent for CAD services. 8/8/2023 0 Comments Grell x sebastian![]() You found nothing of any interest, until you came across several large stacks of crates. Pistol in hand, for precaution’s sake, you carried out your search quickly and efficiently. You had split up to search the main floor, you insisting that Sebastian stayed with and protected Ciel, given that you were something of an old hand at this. Not so much as a scrap of paper to be used as evidence. The warehouse was empty when you got there no smuggled goods, no people who were smuggling them. But your downfall came in not realising they had their own network of eyes and ears. It was perfect really - they could take a boat by night to pick up and drop off their goods and nobody was any the wiser. Their current storage unit was hidden in an abandoned warehouse at the dodgy end of the Thames, nestled between two disused docks. The circle dared not let them escape, lest they hand over any information to the authorities and disrupt the entire ongoing operation. The people being murdered were those who had tried to escape from the ring, bound only to it by their predecessors’ decisions to join it. The people you were chasing were a notorious circle of smugglers who excelled at their craft and whose organisation had been doing so for the last century. “We’re here for information about your most recent guests, the police are refusing to hand anything over.” After a good twenty minutes of trying to make him laugh, Sebastian finally succeeded and you were once again on your way, this time with both a lead and destination in mind. ![]() Your reaction was enough to make him giggle, which Ciel simply tutted at. As it turned out, you would be the one to almost collapse in terror when the coffin propped up on the wall behind you slowly swung open, revealing someone dressed as though he were attending a funeral standing inside it. “Undertaker, are you here?” He called, glancing around for the man in question. The room was so dark you could hardly see anything, but the earl didn’t seem concerned. Sebastian held open the door for you both to enter and you thanked him as you walked through, ignoring the chill curling up your spine at the creaking of the old hinges. You raised a questioning eyebrow at Ciel, who dismissed your confusion with a light wave of his hand. There is only one other person I know who will help us with this.” A few minutes later, you were standing in front of an out-of-the-way shop, the sign of which loudly proclaimed ‘Undertaker’. “They appear to have lost their faith in me and as such are using it as an excuse to not cooperate. “The authorities aren’t going to help us with this,” Ciel informed you gravely. The police force had really tightened their ranks around this case and were not giving the earl any more than the bare minimum of information, despite his position as the Queen’s Guard Dog. A recent string of murders were proving more trouble than they were worth and all you kept coming across were dead ends. “So where are we going now?” You were walking next to Ciel through the cobbled streets of London, Sebastian half a step behind. Because Grell had already been through so much, had barely managed to claw himself back from the edge of despair and had continued doing so through finding you - you couldn’t possibly go now. This was some sort of mistake, right? Had to be. How could this possibly happen? Grell’s mouth was dry, his fingers gripping the dreaded black notebook so hard his knuckles were starting to turn white. ❗️Warnings aaaaangst, all the way through. Links to happy Grell stories in my masterlist □ Also, male pronouns for Grell as requested and a gender neutral reader. Hello again!! You said unhappy and you sure have got it! Seriously though, this is completely different to what I normally write and if anyone’s feeling at all bad about themselves, please don’t read this. 8/8/2023 0 Comments Vango airbeam 600The pre-angled AirBeams give the maximum amount of head height throughout the tent and at the sides where the upright sidewalls increase the height within the tent allowing you to fully maximise the internal space. This family air tent is ideal for first-time campers and experienced campers looking for their first air tent. This three-zoned tent (bedrooms, living area and front awning) gives you plenty of living space and the built-in front awning area makes for a fantastic kitchen area. In our opinion, the Vango Stargrove II Air 600xl is one of the best-value family air tents on the market. 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